Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 16;47(2):633–642. doi: 10.3892/ijmm.2020.4820

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Tian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Melatonin inhibits RANKL-induced osteoclastogenesis in Raw264.7 cells. Cathepsin K (A) mRNA and (B) protein expression in Raw264.7 cells cultured for 7 days with RANKL (100 ng/ml) and M-CSF (30 ng/ml) in the presence of varying concentrations (0.1 or 1 µmol) of melatonin. (C) Tartrate-resistant acid phosphatase activity in Raw264.7 cells cultured for 7 days with RANKL (100 ng/ml) and M-CSF (30 ng/ml) in the presence of varying concentrations (0.1 or 1 µmol) of melatonin. Magnification, ×200 (upper panels) and ×400 (bottom panels). (D) Viability of Raw264.7 cells in the presence of varying concentrations (0.1, 1 or 10 µmol) of melatonin. Cell viability was expressed as OD value. Data are represented as the mean ± SD (n=3). ^**P<0.01. OD, optical density; RANKL, receptor activator of nuclear factor-κB ligand; M-CSF, macrophage colony-stimulating factor.