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. 2020 Dec 16;47(2):633–642. doi: 10.3892/ijmm.2020.4820

Figure 4.

Figure 4

Rev-erbα activation increases the inhibitory effect of melatonin on Raw264.7 cell osteoclastogenesis, whereas the inhibition of Rev-erbα produces the opposite effect. Viability of Raw264.7 cells in the presence of varying concentrations of (A) SR9009 and (B) SR8278 (5, 10 and 15 µmol). Cell viability was expressed as OD value. Rev-erbα and cathepsin K expression in Raw264.7 cells cultured for 7 days with RANKL (100 ng/ml) and M-CSF (30 ng/ml), and for 48 h with 1 µmol melatonin in the presence of varying concentrations of (C) SR9009 and (D) SR8278 (5, 10 and 15 µmol). Data are represented as the mean ± SD (n=3). #P<0.05 and ##P<0.01. OD, optical density; RANKL, receptor activator of nuclear factor-κB ligand; M-CSF, macrophage colony-stimulating factor; Rev-erbα/NR1D1, nuclear receptor subfamily 1 group D member 1.