Figure 1.

Bioinformatics analysis of RNA-Seq results in LPS-stimulated MG63 cells. (A and B) Differential expression level of RNAs in a heatmap and volcano plot. (A) Red color indicates relative overexpression, while blue color indicates relative underexpression. (B) Red indicates upregulated, green indicates downregulated, and black indicates non-statistically significant altered genes. The horizontal dashed line indicates a statistical threshold corresponding to an adjusted P-value of <0.05; x-axis, mRNA log₂ fold change; y-axis, P-value in negative log₁₀ scale. (C and D) Enriched pathway in KEGG analysis. (E) Functional genes network in reactome analysis. Red indicates upregulated, and blue indicates downregulated genes. Main types of functional biological processes are the cell cycle, MAPK activation and ncRNA processing. (F) Each line in the middle of the illustration represents a gene and its rank in GSEA enrichment analysis. The enrichment score indicates the enrichment level of genes in the target gene lists. NES, normalized enrichment score; LPS, lipopolysaccharide.