Table II.

Yield of small RNA and miRNA extracted from plasma and the different EV subpopulations derived from plasma of either controls or PD patients.

	Controls
	Plasma	LEVs	MEVs	SEVs	Pure SEVs
Small RNA (ng)	2.26±1.27	4.32±1.04	4.90±0.95	10.34±3.51	14.51±8.03
miRNA (ng)	0.82±0.49	1.70±0.45	2.12±0.26	4.16±1.17	9.87±6.72
miRNA/small RNA (%)	29.67±5.90	37.07±3.45	39.27±1.83	39.43±1.82	51.37±12.64
	PD patients
	Plasma	LEVs	MEVs	SEVs	Pure SEVs
Small RNA (ng)	8.37±4.32	13.15±2.15	16.18±3.94	11.76±3.94	19.51±4.10
miRNA (ng)	4.03±2.14	5.38±0.96	7.00±1.48	4.74±1.48	8.92±2.40
miRNA/small RNA (%)	32.80±2.40	35.15±0.25	36.50±0.60	35.30±0.60	35.15±0.35

Values are mean ± SEM, n=3 for controls and n=2 for PD patients. Yield of small RNA and miRNA is calculated starting from the concentration (ng/µl) determined by the Small RNA Assay (Agilent). LEVs, large extracellular vesicles; MEVs, medium extracellular vesicles; SEVs, small extracellular vesicles.