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. 2021 Jan 1;18(3):744–755. doi: 10.7150/ijms.48492

Figure 4.

Figure 4

Effects of indoxyl sulfate (IS) on osteogenic differentiation potential of MSCs: Bar graph demonstrate the expression levels of osteogenic-associated genes; RUNX2, OPN, and COL1A1 at day 7 and 14 of differentiation after treatment with indoxyl sulfate relative to control (A). Alkaline phosphatase activity assessed after treatment with indoxyl sulfate (25, 50, 100, and 200 µg/ml) for 7 day and 10 day of differentiation (B). The data were presented as relative ALP activity (U/ml) from four individual experiment (N=4). * P value < 0.05 comparison with control. Alizarin red S staining was assessed for calcium deposition at day 14 of differentiation (C) (Scale bar: 200 µm).