Figure 5.
Effect of Raw 264.7 secretome in TH (A), PNMT (B) and PPARγ (C) gene expression by 3T3-L1 cells. 3T3-L1 preadipocytes and mature adipocytes were incubated for 24 h with CM from both Raw 264.7 macrophages, LPS-activated RAW 264.7 macrophages and untreated (Control) (n=9). *p < 0.05 preadipocytes vs mature adipocytes; #p < 0.05 preadipocytes treated vs preadipocytes control. TH: tyrosine hydroxylase; PNMT: phenylethanolamine-N-methyltransferase; PPARγ: peroxisome proliferator-activated receptor gamma.