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. 2021 Jan 1;18(3):582–592. doi: 10.7150/ijms.52219

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Effect of 3T3-L1 preadipocytes and mature adipocytes secretome in both Raw 264.7 macrophages (A) and LPS-activated RAW 264.7 macrophages (B) viability and in TH (C) and PNMT (D) gene expression by non- and activated Raw 264.7 cells. Raw 264.7 cells were incubated for 24 h with CM from both 3T3-L1 preadipocytes and adipocytes and untreated (Control) (n=6 and 9) after a treatment of 24 h. Results represent the percentage of viability or proliferating cells normalized over the absorbance of control (#p < 0.05 RAW 264.7 macrophages vs activated RAW 264.7 macrophages). TH: tyrosine hydroxylase; PNMT: phenylethanolamine-N-methyl transferase.