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. 2021 Jan 1;18(3):615–625. doi: 10.7150/ijms.51589

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MiR-34c activates and proliferates HSC by targeting ACSL1. (A) Selected interference vectors pLKD-CMV-G&PR-U6-shRNA vector map. (B) Lentivirus infected HSC-T6 cells, screened for fluorescence of stable strains after 3 and 14 days. (C) ACSL1 mRNA expression in each group was reduced by: 57.76%, shR1 (P < 0.05); shR2 decreased by 57.67% (P < 0.05); and shR3 decreased by 34.49% (P < 0.05). (D) ACSL1, α-SMA, and Col1α protein expression in each group. The internal reference is GAPDH. (E) Gray scale analysis of the automated chemiluminescence imaging system indicated that the expression of ACSL1 in the shR1+miR-34c inhibitor group increased 1.49-fold, and the expression of α-SMA decreased by 18.22% and the expression of Col1α decreased by 2.58%.