Fig. 3.

Promoter activities in CbB3 and CbB3-evo1 strains grown on glucose (a and b) and growth of CbB3 PacpP::lacand CbB3 PtonB::lacstrains in Cb-based M9 medium (c). Cells (a ​and ​b) were cultured in M9 0.2% CASA 0.2% glucose at 37 ​°C, and growth and fluorescence signal were monitored in a Tecan Spark plate reader. ​a. ​Optical density at 600 nm. ​b. ​Fluorescence level of GFP (excitation 488 nm / emission 521 nm). CbB3 (dotted line) or CbB3-evo1 (filled line) transformed by pUA66 (black), pUA-Plac (red), pUA-PacpP (blue), pUA-Pgmk (orange), pUA-PmscS (light green), pUA-PtonB (purple), or pUA-PrrnB (dark green). The mean of the data obtained on three culture replicates is shown. ​c. ​CbB3-evo1 (green), CbB3 PacpP::lac (blue), and CbB3 PtonB::lac (purple) strains were cultured in M9 medium containing 0.5% Cb at 37 ​°C. The mean of the data obtained on three culture replicates is shown. Bars indicate the standard deviations. ​(For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)