Fig. 5.

Growth of strains carryinglamBmutations in Cb-based M9 medium (a and b)and promoter activities in CbB3-evo1lamB∗strain grown on Cb (c and d). a. Culture in M9 containing 0.5% Cb at 37 ​°C of the strains CbB3evo1 (blue, dotted line), CbB3evo1 yfeN_S121P ​(blue), CbB3evo1 ​lamB∗ (green), CbC2 (purple, dotted line), CbC2evo1 (red, dotted line), CbC2 ​yfeN_S121P (yellow), and CbC2 lamB∗ (orange). The growth was monitored in Tecan Spark plate reader, and the data show the mean of three culture replicates. ​b. Culture in M9 containing 0.5% Cb at 37 ​°C of the strains CbB3-evo1 (blue) and CbB3-evo1 DlamB ​(orange). The mean of the data obtained on three culture replicates is shown.c. and d. The strains CbB3-evo1 ​lamB∗ ​transformed with pUA66 (black), pUA-Plac (red), pUA-PacpP (blue), pUA-Pgmk (orange), pUA-Ppdc (green) were grown in M9 containing 0.5% Cb at 37 ​°C. Growth and fluorescence signal were monitored in Tecan Spark plate reader. The mean of the data obtained on three culture replicates is shown. c. ​Optical density at 600 ​nm. ​d. Fluorescence level of GFP (excitation 488 nm / emission 521 nm). ​(For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)