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. 2021 Jan 1;12(4):1231–1239. doi: 10.7150/jca.46971

Figure 3.

Figure 3

Identification of ULK1 as a direct target of miR-1262 in GCA. (A) The negative expression correlation between ULK1 mRNA and miR-1262 in gastric cancer tissues. (B) qRT-PCR validation of ULK1 mRNA of miR-1262 in MGC80-3, HGC-27 and OE33 cells transfected with miR-1262 mimics, miR-1262 inhibitors or NC RNA. (C) miR-1262 could significantly inhibit ULK1 protein expression in MGC80-3, HGC-27 and OE33 cells. (D) Relative miR-1262 expression in MGC80-3, HGC-27 and OE33 cells. (E) Schematic constructions of ULK1-WT and ULK1-MU. (F) MGC80-3, HGC-27 and OE33 cells were co-transfected with ULK1-WT and miR-1262 mimics or ULK1-MU and miR-1262 mimics. Luciferase activity was detected at 48h after transfection and normalized relative to the Renilla luciferase expression. All results of the mean of triplicate assays with standard deviation of the mean are presented. *P< 0.05, **P< 0.01, ***P< 0.001.