Figure 6.

Western blot (A-H) and qPCR (I-K) analyze the effects of DS, BRU and their combination on the protein and mRNA expression of Nrf2, HO-1 and VEGF in HGC-27 cells under hypoxic conditions. (A,C-E) HGC-27 cells were treated with a gradient concentration of BRU (0 nM,10 nM, 20 nM, 40 nM, 80 nM, 100 nM) for 24 h. (B,F-K) HGC-27 cells were treated with untreated, 0.1% DMSO, 0.3% DS, 40 nM BRU and 0.3% DS + 40 nM BRU for 24 h, respectively. ns, no significance. * P <0.05, ** P <0.01, *** P < 0.001.