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. 2021 Jan 1;12(4):1169–1178. doi: 10.7150/jca.50512

Figure 4.

Figure 4

Effect of CyH on the expression of stemness-related markers in NSCLC cells. (A-C) NSCLC cells were treated with CyH at 1.51 µM for 10 days. Afterwards, the stem-like sphere formation was observed and the number of spheres was counted under fluorescence microscope (magnification, ×200). The results are representative of three independent experiments (A:549, B:NCI-H460). The number of stem-like spheres (C). (D,E) NSCLC cells were respectively treated with different concentrations (0, 1.51, 3.13, 6.25, 12.5 and 25 µM) of CyH for 16 h. Afterwards, OCT-4, SOX-2, and Nanog protein levels were analyzed by western blotting (D:A549 cells, E:NCI-H460 cells; up: the representative results of three independent experiments, down: the results of density for three independent experiments). (F,G) OCT-4, SOX-2, and Nanog mRNA levels were determined by RT-qPCR. All results represented mean ± SD from three replicate experiments. Compared with control (0 µM), *P<0.05.