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. 2021 Jan 1;12(4):1169–1178. doi: 10.7150/jca.50512

Figure 5.

Figure 5

Effect of CyH on the activation of YAP/TAZ signaling pathway in NSCLC cells. NSCLC cells (A549 and NCI-H460) were respectively treated with different concentrations (0, 1.51, 3.13, 6.25, 12.5 and 25 µM) of CyH for 16 h, followed by the analysis of expression of YAP/TAZ signaling-related factors. (A,B) YAP, p-YAP, TAZ, LATS1/2, and MST1/2 protein levels were analyzed by western blotting (A:A549 cells, B:NCI-H460 cells). (C,D) YAP, p-YAP, TAZ, LATS1/2, and MST1/2 mRNA levels were determined by RT-qPCR. The results represented mean ± SD from three replicate experiments. Compared with control (0 µM), *P<0.05, **P<0.01. (E) The interaction between YAP and TEAD was detected by co-immunoprecipitation.