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. 2021 Jan 1;11(5):2364–2380. doi: 10.7150/thno.47996

Figure 1.

Figure 1

Human BMSC-derived sEVs can be taken by MM cells. (A) Transmission electron microscopy images of human BMSC-derived sEVs. (B) Size distribution of human BMSC-derived sEVs was determined using the nanoparticle tracking analysis. (C) Exosomal positive markers, including CD63, CD9, flotillin-1, as well as a negative marker calreticulin, in BMSC and BMSC-derived sEV lysate were measured using western blot. (D) 50 μg/mL DID-labeled BMSC-derived sEVs or DID control solution was added to four MM cell lines, including RPMI 8226, MM1S, U266, and H929. After 24 h of culture, the fluorescence signal of DID in these cells was examined using flow cytometry. PBS was added to cells and included as a control. (E) 50 μg/mL DID-labeled BMSC-derived sEVs were added to four MM cell lines and the mean and median fluorescence intensity of DID in these cells was determined using flow cytometry after the culture for indicated times. n=3. Error bar, mean ± SD.