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. 2021 Jan 1;11(5):2364–2380. doi: 10.7150/thno.47996

Figure 5.

Figure 5

Inhibitors of macropinocytosis impair the uptake of BMSC-derived sEVs by MM cells. (A) MM1S cells pre-treated with EIPA, amiloride, wortmannin, or omeprazole at the indicated concentrations for 30 min were cultured with 50 μg/mL DID-labeled BMSC-derived sEVs for another 4 h. The mean fluorescence intensity of DID in these cells was determined using flow cytometry. (B) MM1S cells were treated with EIPA, amiloride, wortmannin, or omeprazole at the indicated concentrations for 4.5 h in the absence of sEVs and their cell viability was measured. (C) RPMI 8226, U266, or H929 cells were treated with EIPA, amiloride, or wortmannin at the indicated concentration for 4.5 h in the absence of sEVs and their cell viability was measured. (D) RPMI 8226, (E) U266, or (F) H929 cells were pre-treated with EIPA, amiloride, or wortmannin at the indicated concentration for 30 min and cultured with 50 μg/mL DID-labeled BMSC-derived sEVs for another 4 h. The mean fluorescence intensity of DID in these cells was determined using flow cytometry. One-way ANOVA followed by multiple compressions was used for comparing multiple groups. n=3. Error bar, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.