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. 2021 Jan 1;11(5):2364–2380. doi: 10.7150/thno.47996

Figure 7.

Figure 7

Dynasore inhibits sEV uptake in primary MM cells. (A-F) BMMCs obtained from 5 MM patients were pre-treated with or without dynasore at different final concentrations (12.5, 25, or 50 µM) for 30 min and then incubated with 50 μg/mL DID-labeled sEVs for another 4 h. After staining with anti-mouse CD45-PE, anti-mouse CD38-Pacific Blue, and Annexin V-FITC, cells were acquired using flow cytometry. Live BM cells (Annexin V-) were gated and analyzed. (A) Gating of MM (CD45-CD38+) and CD45+ immune cells from MM patient 1 (left panel). Representative flow cytometry plots showing the DID signal in MM patient 1 MM and immune cells treated with or without dynasore. (B) Median florescence intensity of DID in total, MM, and immune cells was analyzed and presented by histograms. Same analyses were performed using BMMCs isolated from MM patient (C) 2, (D) 3, (E) 4, and (F) 5. One-way ANOVA followed by multiple compressions was used for comparing multiple groups. Error bar, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.