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. 2021 Jan 1;11(5):2424–2441. doi: 10.7150/thno.46873

Figure 5.

Figure 5

NLRP3 NBD and LRR domains are involved in NLRP3-PPARγ interaction. (A) Schematic diagrams show NLRP3 LXXLL mutants (ΔLRRLXXLL, mLRR, and mNBD). (B) Immunoprecipitation and immunoblot analysis of the interaction between NLRP3 LXXLL mutants and PPARγ in HEK293T cells. (C) Schematic diagrams show NLRP3 truncated mutants (ΔPYD, ΔNBD, and ΔLRR). (D) Immunoprecipitation and immunoblot analysis of the interaction between NLRP3 truncated mutants and PPARγ in HEK293T cells. (E) Schematic diagrams show NLRP3 truncated deletions to retain only PYD, NBD, and LRR. (F-H) Immunoprecipitation and immunoblot analysis of the interaction between NLRP3 truncated deletions and PPARγ in HEK293T cells. LRR, leucine-rich repeat domain; NBD, nucleotide-binding domain; PYD, pyrin domain. PPARγ and truncated NLRP3 bands are labeled with arrowhead. Representative blots in (D), (F), (G), and (H) are from three independent experiments; and in (B) are from two independent experiments. Experiment replicates are shown in Figure S14.