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. 2021 Jan 1;11(5):2077–2078. doi: 10.7150/thno.56462

Figure 4.

Figure 4

The promotion of cell migration, invasion and cancer metastasis after Twist overexpression primarily depends on ROR1. (A-B) The indicated molecules were analyzed by Western blot in the Hs578T and MDA-MB-231 cells stably expressing control, ROR1 shRNA, Twist, or both, as indicated. (C-F) Cell migration and invasion were determined in the indicated stable cell lines. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 using Student's t-test. (G-H) An in vivo lung metastasis model was established in nude mice using MDA-MB-231 cells stably expressing control, ROR1 shRNA, Twist, or both, as indicated. (G) Representative results of gross and H&E staining (middle scale: 40×; right scale: 200×) of metastatic lung nodules. Scale bars, 40×,500μm; 200×,100μm. (H) Illustration of the statistical results (n = 6). The results are expressed as the mean ± SD. * P < 0.05, *** P < 0.001 using Student's t-test.