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. 2021 Jan 1;11(5):2410–2423. doi: 10.7150/thno.47182

Figure 5.

Figure 5

TRPV4 siRNA abolished calcium oscillations and VSMC wound closure. (A) Western blot results indicate the interference efficiencies of 3 TRPV4 siRNA sequences. (B) Time-lapse FRET images of the chances in cytoplasmic calcium in VSMCs transfected with TRPV4 si-1-Cy3 or si-NC during the PMV treatment process. The hot and cold colors represent high and low FRET ratios, indicating high and low levels of cytoplasmic calcium change, respectively. Scale bar: 30 μm. (C) The time courses represent the normalized FRET/ECFP ratio averaged over the cell body in VSMCs transfected with TRPV4 si-1-Cy3 (n = 16) or si-NC (n = 13) after PMV treatment, and all the shadowed areas indicate the S.E.M. Comparation of the max normalized FRET/ECFP ratio (D) and frequency of the cytoplasmic calcium oscillations (E) between the VSMCs transfected with TRPV4 si-1-Cy3 (n = 16) or si-NC control (n = 13) after PMV treatment. (F) The migration of VSMCs transfected with TRPV4 si-1 or si-NC control after 24 h of PMV treatment. Scale bar: 200 μm. (G) The histogram shows the fold change in the level of TRPV4 si-1-transfected VSMC migration relative to that of the si-NC control-transfected VSMC migration. The values are shown as the mean ± S.E.M. for each condition (n = 6). ** P < 0.01, **** P < 0.0001.