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. 2021 Jan 11;29(1-2):41–54. doi: 10.1038/s41434-020-00214-w

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© The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Medium only without PBMCs yielded zero counts. Medium with DMSO (mock control) consistently demonstrated ≤10 SFU/well across all monkeys. Positive responses were observed for PHA stimulation across all monkeys, which served as a pan-T cell stimulation control for the functionality of PBMCs. Three monkeys (36, 37, 38) responded positively when stimulated with monkey CMV peptide pools (UL55 and/or UL83); no or low responses were observed in any monkey when stimulated with a human CMV peptide pool (pp65) or other human virus-derived peptide pools (CEF).