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. 2021 Jan 11;29(1-2):41–54. doi: 10.1038/s41434-020-00214-w

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© The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature 2021

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Fig. 3 — PBMCs from three positive and one negative responders were stimulated with two CMV peptide pools (UL55 and UL83) in three different runs (two sets of triplicates per run) and responses were measured as SFU/well. A Intra-triplicate %CVs were plotted against intra-triplicate mean SFU/well responses following UL55 (blue dots) and UL83 (red dots) peptide pool stimulation. B Intra assay %CVs were plotted against intra-assay mean SFU/well responses following UL55 (blue dots) and UL83 (red dots) peptide pool stimulation. C Inter-assay %CVs were plotted against the inter-assay mean SFU/well responses following UL55 (blue dots) and UL83 (red dots) peptide pool stimulation. In all panels, the horizontal dotted lines represent the pre-defined acceptable %CV limit, and the vertical dotted line represents the preliminary LOD value (10 SFU/well).