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. 2021 Jan 11;29(1-2):41–54. doi: 10.1038/s41434-020-00214-w

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© The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — PBMCs collected after AAV5 gene therapy adminstration were left unstimulated (mock) or stimulated with various peptide pools or PHA and tested in INFγ ELISpot. Respones were expressed as SFU/million PBMCs. A Cohort 1: PBMCs from 18 animals collected on Day 13 (black dots) and 28 (blue dots) after AAV5 gene transfer. B Cohort 2: PBMC samples from 18 animals collected on Day 13 (black dots) and 56 (red dots) after AAV5 gene transfer. PHA responses reported ‘as too many to count’ (TNTC) were imputed as 4000 SFU/million PBMC for graphical purposes.