Table 2.

Validation of assay precision using UL83 and UL55 stimulation.

Monkey ID	Peptide pool	Analyst	Run	Triplicates	Intra-assay		Inter-assay		Inter-analyst
				Mean SFU/well	%CV	Average triplicate %CV	Mean SFU/well	%CV	Mean SFU/well	%CV
10	UL83	A	5	80	12.9	10.9	74	9.9	78	6.3
			6	79	11.2
			10	74	14.3
		B	9	60	6.1				71
			11	75	8.2
			13	78	12.9
16	UL55	A	5	208	7.8	5.5	212	11.3	214	1.5
			6	197	4.7
			10	238	2.2
		B	9	174	4.2				210
			11	228	7.0
			13	228	6.9
13	UL83	A	5	389	8.3	5.9	405	10.9	433	9.6
			6	480	2.4
			10	429	5.3
		B	9	372	4.1				378
			11	358	6.0
			13	404	4.1

PBMCs from three CMV-responsive monkeys (low, medium and high responders) were stimulated with monkey CMV peptide pools UL83 or UL55 in six different runs per monkey. Data were generated by two analysts, with three runs per analyst on three different days.