FIGURE 1.

Presence of subretinal fibrosis in very low–density lipoprotein receptor (Vldlr)^−/− retinas. (A) Representative H&E staining of eyecups from wild-type (WT) and Vldlr ^−/− mice. (B) Masson staining of collagen (blue) in the eyecups of WT mice and Vldlr ^−/− mice. Representative images of immunostaining of (C) vimentin, (D) alpha-smooth muscle actin (α-SMA), and (E) collagen-1 in the eyecups of WT mice and Vldlr ^−/− mice. (F) Protein levels of vimentin, α-SMA, collagen-1, and fibronectin in the eyecups of WT mice and Vldlr ^−/− mice were measured by Western blot analysis. Levels of (G) vimentin, (H) α-SMA, (I) collagen-1, and (J) fibronectin were quantified by densitometry and normalized to GAPDH levels (mean ± SEM; n = 8. ***p < 0.001).