Figure 8.

Time courses of association of RAB 5, 7, or 11 with EGFR-containing endosomes. (a) Representative low- and high-magnification and split-color confocal micrographs of EGFR-containing endosomes (EGFR, Alexa Fluor 594, false-colored green) costained with RAB5, RAB7, or RAB11 (Alexa Fluor 488, false-colored red), as indicated, at the indicated times after initiating EGFR trafficking. Arrowheads indicate colocalization. (b) Time course of colocalization of EGFR-containing endosomes with RAB5, RAB7, or RAB11 quantified from confocal micrographs; mean ± SEM; n > 30 cells per time point.