Reagent	Final concentration	Amount per sample
LTM in DMSO, store at −80°C	4 mM	depending on your organism
Triton, store at 20°C	20%	300 μL
SDS, store at 20°C	20%	100 μL
DTT, store at −20°C	1 M	15 μL
EtOH, store at −20°C	80%	750 μL
CHX in EtOH, store at −20°C	50 mg/mL	100 μL
NaOH, store at 20°C	1 M	60 μL
HCl, store at 20°C	1 M	2 μL
Tris-HCl pH 7, store at 20°C	10 mM	1 mL
Tris-HCl pH 8, store at 20°C	10 mM	20 μL
Tween20, store at 20°C	10%	100 μL
NaCl, store at 20°C	3 M	200 μL