| Antibodies |
|
| PE Rat Anti-CD11b |
BD |
553311 |
| APC anti-mouse F4/80 antibody |
BioLegend |
123116 |
| 7-AAD |
BD |
559925 |
| Phospho-Akt (Ser473) Rabbit mAb |
Cell Signaling |
9271 |
| Phospho mTOR antibody |
Cell Signaling |
2971 |
| ECL anti-rabbit IgG horseradish peroxidase lined whole antibody |
GE |
NA934-1ML |
| GAPDH rabbit monoclonal antibody |
Cell Signaling |
2118 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Recombinant mouse IL-13 |
R and D |
413-ML-005 |
| Recombinant mouse CSF-1 |
Gibco |
PMC2044 |
| DMEM (high glucose) |
Gibco |
5796 |
| Stain buffer |
BD |
554656 |
| FBS |
Biowest |
S1650 |
| Penicillin streptomycin |
Gibco |
10378016 |
| PBS tablets |
Takara |
T900 |
| 0.05% Trypsin-EDTA |
Gibco |
25300-054 |
| Cell lysis buffer |
CST |
9803 |
| Complete protease inhibitor cocktail |
Roche |
11697498001 |
| 4× Laemmli sample buffer |
Bio-Rad |
1610747 |
|
| Experimental models: organisms/strains |
|
| C57BL6/J |
Jackson Laboratory |
664 |
|
| Oligonucleotides |
|
| IL-10 Fw GCTCTTACTGACTGGCATGAG |
N/A |
N/A |
| IL-10 Rv CGCAGCTCTAGGAGCATGTG |
N/A |
N/A |
| TNF-α Fw CTGAACTTCGGGGTGATCGG |
N/A |
N/A |
| TNF-α Rv GGCTTGTCACTCGAATTTTGAGA |
N/A |
N/A |
| Arg1 Fw CTCCAAGCCAAAGTCCTTAGAG |
N/A |
N/A |
| Arg1 Rv GGAGCTGTCATTAGGGACATCA |
N/A |
N/A |
|
| Software and algorithms |
|
| Prism 7 |
GraphPad |
N/A |
| MS Excel |
Microsoft |
N/A |
| Photoshop CC |
Adobe |
N/A |
|
| Other |
|
| Cyclophillin probe |
Applied Biosystems |
4316034 |
| Cell strainer (100 μm) |
Falcon |
352360 |
| Lysing buffer |
BD |
555899 |
| Cell scraper |
IWAKI |
9000-220-CS |
| RNeasy mini kit |
QIAGEN |
74106 |
| Round bottom polystyrene tubes |
Falcon |
352058 |