Antibodies |
|
PE Rat Anti-CD11b |
BD |
553311 |
APC anti-mouse F4/80 antibody |
BioLegend |
123116 |
7-AAD |
BD |
559925 |
Phospho-Akt (Ser473) Rabbit mAb |
Cell Signaling |
9271 |
Phospho mTOR antibody |
Cell Signaling |
2971 |
ECL anti-rabbit IgG horseradish peroxidase lined whole antibody |
GE |
NA934-1ML |
GAPDH rabbit monoclonal antibody |
Cell Signaling |
2118 |
|
Chemicals, peptides, and recombinant proteins |
|
Recombinant mouse IL-13 |
R and D |
413-ML-005 |
Recombinant mouse CSF-1 |
Gibco |
PMC2044 |
DMEM (high glucose) |
Gibco |
5796 |
Stain buffer |
BD |
554656 |
FBS |
Biowest |
S1650 |
Penicillin streptomycin |
Gibco |
10378016 |
PBS tablets |
Takara |
T900 |
0.05% Trypsin-EDTA |
Gibco |
25300-054 |
Cell lysis buffer |
CST |
9803 |
Complete protease inhibitor cocktail |
Roche |
11697498001 |
4× Laemmli sample buffer |
Bio-Rad |
1610747 |
|
Experimental models: organisms/strains |
|
C57BL6/J |
Jackson Laboratory |
664 |
|
Oligonucleotides |
|
IL-10 Fw GCTCTTACTGACTGGCATGAG |
N/A |
N/A |
IL-10 Rv CGCAGCTCTAGGAGCATGTG |
N/A |
N/A |
TNF-α Fw CTGAACTTCGGGGTGATCGG |
N/A |
N/A |
TNF-α Rv GGCTTGTCACTCGAATTTTGAGA |
N/A |
N/A |
Arg1 Fw CTCCAAGCCAAAGTCCTTAGAG |
N/A |
N/A |
Arg1 Rv GGAGCTGTCATTAGGGACATCA |
N/A |
N/A |
|
Software and algorithms |
|
Prism 7 |
GraphPad |
N/A |
MS Excel |
Microsoft |
N/A |
Photoshop CC |
Adobe |
N/A |
|
Other |
|
Cyclophillin probe |
Applied Biosystems |
4316034 |
Cell strainer (100 μm) |
Falcon |
352360 |
Lysing buffer |
BD |
555899 |
Cell scraper |
IWAKI |
9000-220-CS |
RNeasy mini kit |
QIAGEN |
74106 |
Round bottom polystyrene tubes |
Falcon |
352058 |