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. 2020 Dec 31;2(1):100246. doi: 10.1016/j.xpro.2020.100246
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

PE Rat Anti-CD11b BD 553311
APC anti-mouse F4/80 antibody BioLegend 123116
7-AAD BD 559925
Phospho-Akt (Ser473) Rabbit mAb Cell Signaling 9271
Phospho mTOR antibody Cell Signaling 2971
ECL anti-rabbit IgG horseradish peroxidase lined whole antibody GE NA934-1ML
GAPDH rabbit monoclonal antibody Cell Signaling 2118

Chemicals, peptides, and recombinant proteins

Recombinant mouse IL-13 R and D 413-ML-005
Recombinant mouse CSF-1 Gibco PMC2044
DMEM (high glucose) Gibco 5796
Stain buffer BD 554656
FBS Biowest S1650
Penicillin streptomycin Gibco 10378016
PBS tablets Takara T900
0.05% Trypsin-EDTA Gibco 25300-054
Cell lysis buffer CST 9803
Complete protease inhibitor cocktail Roche 11697498001
4× Laemmli sample buffer Bio-Rad 1610747

Experimental models: organisms/strains

C57BL6/J Jackson Laboratory 664

Oligonucleotides

IL-10 Fw GCTCTTACTGACTGGCATGAG N/A N/A
IL-10 Rv CGCAGCTCTAGGAGCATGTG N/A N/A
TNF-α Fw CTGAACTTCGGGGTGATCGG N/A N/A
TNF-α Rv GGCTTGTCACTCGAATTTTGAGA N/A N/A
Arg1 Fw CTCCAAGCCAAAGTCCTTAGAG N/A N/A
Arg1 Rv GGAGCTGTCATTAGGGACATCA N/A N/A

Software and algorithms

Prism 7 GraphPad N/A
MS Excel Microsoft N/A
Photoshop CC Adobe N/A

Other

Cyclophillin probe Applied Biosystems 4316034
Cell strainer (100 μm) Falcon 352360
Lysing buffer BD 555899
Cell scraper IWAKI 9000-220-CS
RNeasy mini kit QIAGEN 74106
Round bottom polystyrene tubes Falcon 352058