| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Merck | T5648-1G |
| Isoflurane inhalation solution | Pfizer | n/a |
| Ibuprofen | Wako, Japan | 094-02643 |
| Xylocaine injection 1% with epinephrine (lidocaine) | Aspen Japan | n/a |
| OCT mounting medium | Sakura Finetek | 4583 |
| Vectashield | Vector | H-1000 |
| 4% paraformaldehyde phosphate buffer solution | Nacalai Tesque | 09154-85 |
| Sucrose | Nacalai Tesque | 30404-45 |
| Sunflower oil | Sigma | 1642347 |
| Corn oil | Sigma | C8267 |
| Flunixin meglumine | Fujita | FLUNIXIN INJ. 10% |
| Antibiotics (sulfadiazine/trimethoprim) | Kyoritsu Seiyaku | Tribrissen injection |
| Tarivid ophthalmic ointment 0.3% (Ofloxacin) | Santen Pharmaceutical | 1319722M1056 |
| Experimental models: organisms/strains | ||
| Mice: C57BL/6-Tg(Nes-cre/ERT2)KEisc/J | The Jackson Laboratory | 016261 |
| Mice: Ai38(RCL-GCaMP3) | The Jackson Laboratory | 029043 |
| Software and algorithms | ||
| nVista HD | Inscopix | n/a |
| Inscopix image decompressor | Inscopix | n/a |
| EEG and EMG recording and analysis software | KISSEI COMTEC | Vital Recorder and Sleep Sign |
| Mosaic | Inscopix | n/a |
| MATLAB | MathWorks | 2016b |
| IgorPro 8 | WaveMetrics | n/a |
| Other | ||
| ∗GRIN lens probe, 1.0-mm diameter, ~4.0-mm length | Inscopix | 1050-004595 |
| ∗Rectangular metal frame | Narishige | CF-10 |
| ∗Baseplate | Inscopix | 1050-004638 |
| ∗Miniscope | Inscopix | nVista(2.0) |
| ∗Baseplate cover | Inscopix | 1050-004639 |
| ∗Miniscope gripper | Inscopix | 1050-002199 |
| ∗Dummy miniscope | Inscopix | 1050-003762 |
| ∗∗Electrodes for EEG recording | Yamazaki | ϕ1.0 × 2.0 |
| ∗∗Electrodes for EMG recording | Cooner Wire | AS633 |
| ∗∗Flat cable for EEG/EMG signal transfer | Hitachi Cable | 20528-ST LF |
| ∗∗EEG/EMG signal amplifier | Kyoto Biotex | n/a |
| ∗∗Analog-to-digital convertor for EEG/EMG signal | Contec | AD16-16U(PCIEV) |
| ∗∗4-pin header for EEG/EMG socket | Hirose | A3B-4PA-2DSA(71) |
| Fear conditioning setup | Med Associates | n/a |
| Isoflurane vaporizer | Shinano Seisakusyo | SN-487-OT |
| Stereotaxic frame | Narishige | SR-6M |
| Drill | Minitor | Minimo |
| Vacuum pump | Iwaki | VPUMP-140 |
| Surgical microscope | Leica | Leica GZ6 |
| Dental cement liquid | Shofu | PROVINICE 204310644 |
| Dental cement powder | Shofu | PROVINICE 096017656 |
| Loctite cyanoacrylate adhesive | Loctite | Loctite 454 |
| Super glue | Konishi | Alon Alfa A |
| Silicone | Shofu | DENTSILICONE-V |
| Head-fixing support | Narfishige | MAG-1 |
| Perista perfusion pump | ATTO | AC2110 |
| Cryostat | Leica Microsystems | Leica CM 1850 |
| Confocal fluorescent microscope | Olympus | FV1200 |
| Glass electrodes | Harvard Apparatus | GC150F-7.5 |
| Ultrasonic bath | SND | US-101 |
| Glass electrode puller | Narishige | PC-10 |
| Deposited data | ||
| Data and code underlying the results described in this manuscript | https://github.com/vergaloy/ABNs-Calcium-extraction | n/a |
| Raw data from the related article (Kumar et al., 2020) | https://data.mendeley.com/datasets/gfbdv5kfrz/1 | n/a |
∗Materials only for imaging surgery.
∗∗Materials only for EEG/EMG surgery.