Figure 5.

Dopamine induces formation of functional extracellular traps

(A) BV2 microglia were incubated with dopamine (DA) for 3 hr. FITC-tagged E. coli (green) was added followed by DA incubation, and cells were further incubated for 21 hours. ETs were stained with DAPI (blue). In the overlay image, green dots are overlapping with the blue ETs, suggesting that ETs are trapping E. coli. Images are representative of two experiments. At least 7 frames were imaged per well. Scale bars, 100μm. (B) BV2 microglia were pretreated with N-acetyl-L-cysteine (NAC) and were incubated with dopamine (DA) for 3 hr. FITC-tagged E. coli (green) was added followed by DA incubation, and cells were further incubated for 21 hours. ETs were stained with DAPI (blue). In the overlay image, green dots are overlapping with the blue ETs, suggesting that ETs are trapping E. coli. Images are representative of two experiments. At least 7 frames were imaged per well. Scale bars, 100μm.