Figure 4.

SNHG1 bound to miR-3127-5p. (a) Subcellular location of SNHG1 was assessed using cytoplasmic and nuclear RNA purification, followed by qRT-PCR. (b) The predicted binding site of miR-3127-5p on SNHG1. (c) Luciferase activity in HeLa cells cotransfected with indicated doses of miR-3127-5p mimics and luciferase reporter containing wild-type or miR-3127-5p binding site mutated SNHG1. Results are presented as the relative ratio of firefly to renilla luciferase activity. (d) Luciferase activity in HeLa cells cotransfected with miR-3127-5p inhibitors and luciferase reporter containing wild-type or miR-3127-5p binding site mutated SNHG1. Data are presented as the relative ratio of firefly to renilla luciferase activity. (e) HeLa cell lysates were mixed with SNHG1 antisense biotinylated probes; after pull-down, RNA was enriched and assessed with qRT-PCR. (f) HeLa cell lysates were mixed with biotinylated SNHG1; after pull-down, RNA was enriched and measured with qRT-PCR. Results are presented as mean ± SD. ***P < 0.001, ns: not significant. (A color version of this figure is available in the online journal.)