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. 2020 Sep 3;246(1):20–30. doi: 10.1177/1535370220955139

Figure 6.

Figure 6.

SNHG1 activated Wnt/β-catenin signaling via modulating miR-3127-5p/FZD4. (a) After transient transfection of TOPFlash or FOPFlash plasmids into HeLa cells overexpressing wild-type or miR-3127-5p binding site mutated SNHG1, luciferase activity was assessed and presented as the ratio of TOPFlash/FOPFlash. (b) After transient transfection of TOPFlash or FOPFlash plasmids into HeLa cells silencing SNHG1 or control, luciferase activity was assessed and presented as the ratio of TOPFlash/FOPFlash. (c) Wnt/β-catenin targets expression level in HeLa cells overexpressing wild-type or miR-3127-5p binding site mutated SNHG1 was measured using qRT-PCR. (d) Wnt/β-catenin targets expression level in HeLa cells silencing SNHG1 or control was measured using qRT-PCR. (e) Luciferase activity in HeLa cells overexpressing SNHG1 or control and simultaneous treatment with 50 µM baicalein after transient transfection of TOPFlash or FOPFlash plasmids was measured and presented as the ratio of TOPFlash/FOPFlash. (f) Wnt/β-catenin targets expression level in HeLa cells overexpressing SNHG1 or control and simultaneous treatment with 50 µM baicalein for 48 h was measured using qRT-PCR. Data are presented as mean ± SD. **P < 0.01, ***P < 0.001, ns: not significant. (A color version of this figure is available in the online journal.)