Extended Data Figure 7 |. Overexpressing Cat rescues the ISC death induced by Arf79F^RNAi but not CycT^RNAi expression and dying ISCs activate JNK signalling and autophagy in enterocytes.

a–h, The genotypes of the flies in each panel were: a, esg^ts > Cat, 29 °C, 7 d (n = 25). b, esg^ts > Arf79F^RNAi + Cat, 29 °C, 7 d (n = 32). c, esg^ts > Sod, 29 °C, 7 d (n = 24). d, esg^ts > Arf79F^RNAi + Sod, 29 °C, 7 d (n = 30). e, esg^ts > Sod2, 29 °C, 7 d (n = 22). f, esg^ts > Arf79F^RNAi + Sod2, 29 °C, 7 d (n = 32). g, esg^ts > CycT^RNAi, 29 °C, 7 d (n = 35). h, esg^ts > CycT^RNAi + Cat, 29 °C, 7 d (n = 37). Overexpressing Cat, but not sod or sod2, in stem cells (esg^ts > Arf79F^RNAi + Cat) rescued the stem-cell death induced by Arf79F knockdown but not that induced by CycT knockdown (esg^ts>CycT^RNAi+Cat). i–n, The genotypes of the flies in each panel were: i, puc–lacZ, 29 °C, 7 d (n = 17). j, esg^ts > Arf79F^RNAi + puc–lacZ, 29 °C, 7 d (n = 20). Yellow arrows point to GFP⁺ cells. k, esg > GFP, 29 °C, 4 d (n = 12). l, FRT^82B–γ-COP10 MARCM clones, 4 d (n = 17). Yellow arrows point to GFP⁺ clones. m, esg^ts > lacZ^RNAi + pmCherry–Atg8a, 29 °C, 7 d (n = 22). n, esg^ts > Arf79F^RNAi + pmCherry–Atg8a, 29 °C, 7 d (n = 25). Arf79F knockdown in ISCs induced Puc–lacZ (compare i with j) and Cherry–Atg8a (compare m with n) expression in enterocytes, p-JNK was induced in enterocytes in γ-COP mutant MARCM clones, compare k with l). The posterior midguts of flies with the indicated genotypes were dissected, stained with the indicated antibodies and analysed by confocal microscopy. Scale bars in a–n: 10 μm.