Extended Data Figure 9 |. Knockdown of components of the JNK pathway or engulfment genes in enterocytes blocks the ISC death induced by Arf79F^RNAi or δ-Cop^RNAi expression.

The genotypes of the flies in each panel were: a, NP1^ts esg^ts > lacZ^RNAi, 29 °C, 7 d (n = 20). b, NP1^ts esg^ts > Arf79F^RNAi, 29 °C, 7 d (n = 32). c, NP1^ts esg^ts > δ-Cop^RNAi, 29 °C, 7 d (n = 30). d, NP1^ts esg^ts > Arf79F^RNAi + bsk^DN, 29 °C, 7 d (n = 30). NP1^ts esg^ts > δ-Cop^RNAi + Rac1^DN, 29 °C, 7 d (n = 202). NP1^ts esg^ts > δ-Cop^RNAi + mbc^RNAi, 29 °C, 7 d (n = 18). NP1^ts esg^ts > Arf79F^RNAi + drpr^RNAi, 29 °C, 7 d (n = 32). NP1^ts esg^ts > δ-Cop^RNAi + Atg5^RNAi, 29 °C, 7 d (n = 17). i NP1^ts esg^ts > δ-Cop^RNAi + Atg12^RNAi, 29 °C, 7 d (n = 25). j, NP1^ts esg^ts > δ-Cop^RNAi + PSR^RNAi, 29 °C, 7 d (n = 22). k, NP1^ts esg^ts > Arf79F^RNAi + mys^RNAi, 29 °C, 7 d (n = 35). NP1^ts esg^ts > Arf79F^RNAi + p35, 29 °C, 7 d (n = 27). m, NP1^ts > Jra^Asp (a constitutively activate form of Jun), 29 °C, 7 d (n = 25). n, NP1^ts > drpr, 29 °C, 7 d (n = 20). Expresslon of Jra^Asp and drpr in enterocytes ehmmates Dl⁺ ISCs. The posterior midguts of files with the indicated genotypes were dissected, stamed with the indicated antibodies and analysed by confocal microscopy. White arrows point to Dl⁺ ISCs, yellow arrowheads point to Pros⁺ enteroendocrine cells and green arrows point to enterocytes. Scale bars m a–n: 10 μm.