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Published in final edited form as: Nature. 2016 Sep 28;538(7623):109–113. doi: 10.1038/nature19788

Extended Data Figure 2 | — a, The COPI–lipolysis–β-oxidation pathway. The COPI–Arf1 complex controls lipid homeostasis by regulating adipocyte differentiation-related protein (ADRP), tailinteracting protein of 47kDa (Tip47) and adipocyte triglyceride lipase (ATGL)¹⁵. Triglycerides (TG), diglyceride (DG), fatty acid (FA), Acyl-CoA synthetase (ACS). b–j, The COPI–Arf1 complex regulates stem cell survival. The genotypes of the flies in each panel were: b, esg^ts wg^ts > lacZ^RNAi, 29 °C, 7 d (n = 38). c, esg^ts wg^ts > β-Cop^RNAi, 29 °C, 7 d (n = 23). d, esg^ts wg^ts > δ-Cop^RNAi, 29 °C, 7 d (n = 32). e, esg^ts wg^ts > γ-Cop^RNAi, 29 °C, 7 d (n = 27). f, esg^ts wg^ts > ζ-Cop^RNAi, 29 °C, 7 d (n = 31). g, esg^ts wg^ts > β’-Cop^RNAi, 29 °C, 7 d (n = 29). h, esg^ts wg^ts > garz^RNAi, 29 °C, 7 d (n = 27). i, esg^ts wg^ts > Acsl^RNAi, 29 °C, 7 d (n = 32). j, esg^ts wg^ts >bgm^RNAi, 29 °C, 7 d (n = 25). k, Quantification of GFP⁺ cells in the indicated panels. Data show mean ± s.e.m. Statistical significance was determined by Student’s t-test, ***P < 0.0001. NS, not significant (P >0.05). l–q Knockdown of the COPI–Arf79F complex did not kill differentiated cells. The genotypes of the flies in each panel were: l, n, NP1^ts > lacZ^RNAi, 29 °C, 7 d (n = 25). m, o, NP1^ts > Arf79F^RNAi, 29 °C, 7 d (n = 32). p, tsh^ts >lacZ^RNAi, 29 °C, 7 d (n = 22). q, tsh^ts > Arf79F^RNAi, 29 °C, 7 d (n = 25). r, esg^ts wg^ts > Ras^V12 + ζ-Cop^RNAi, 29 °C, 7 d (n = 27). s, esg^ts wg^ts > N^DN + δ-Cop^RNAi, 29 °C, 7 d (n = 30). t, u, esg^ts > N^DN + Arf79F^RNAi, 29 °C, 7 d (n = 40). v, esg^ts wg^ts > Ras^V12 + Acsl^RNAi, 29 °C, 7 d. (w) esg^ts wg^ts > β-Cop^RNAi + Hnf4, 29 °C, 7 d. x, esg^ts wg^ts > ζ-Cop^RNAi + scu, 29 °C, 7 d. White arrows in b–j and r, s, w, x, point to the hindgut–midgut junction. Yellow arrows point to Pros⁺ enteroendocrine cells in t; green arrows point to Dl⁺ ISCs in t, and a white arrow points to a remaining GFP⁺ stem cell in u. Scale bars in b–j and l–x, 10 μm.