Figure 2. HCP5 knockdown suppressed proliferation, migration, and invasion, and promoted apoptosis in osteosarcoma (OS) cells. A, The expression of HCP5 in MG-63 and U2OS cells transfected with si-NC, si-HCP5#1, si-HCP5#2, or si-HCP5#3 was measured by qRT-PCR. B and C, Cell proliferative capacity was evaluated by MTT assay. D, Cell apoptosis was detected 48 h after transfection by flow cytometry. E and F, Cell migration and invasion were confirmed by transwell assay in MG-63 and U2OS cells (100×, scale bar: 50 μm). Data are reported as means±SD. **P<0.01 (Student's t-test and ANOVA).
