Figure 3. HCP5 directly targeted miR-101. A, The online software miRcode predicted the putative binding sites between HCP5 and miR-101. B, The expression of miR-101 was exhibited in osteosarcoma (OS) tissues and adjacent normal tissues. C, Correlation between the expression of HCP5 and miR-101 in OS tissues. D, The expression of miR-101 was measured in OS cell lines MG-63 and U2OS and normal cells hFOB 1.19. E and F, Luciferase reporter assay was performed to confirm the interaction between HCP5 and miR-101 in MG-63 and U2OS cells. G and H, Relative expression of miR-101 was examined in MG-63 and U2OS cells transfected with si-NC (negative control), si-HCP5#2, vector NC, or HCP5. Data are reported as means±SD. **P<0.01 (Student's t-test and ANOVA).
