Figure 4. Depletion of miR-101 could reverse the impact on cell proliferation, apoptosis, migration, and invasion caused by HCP5 knockdown in osteosarcoma (OS) cells. A, The expression of miR-101 in MG-63 and U2OS cells transfected with si-NC (negative control), si-HCP5#2, si-HCP5#2+miR-NC, or si-HCP5#2+miR-101 inhibitor was measured by qRT-PCR. B and C, Cell proliferation was assessed at 24, 48, 72, and 96 h after transfection by MTT assay in MG-63 and U2OS cells. D, Cell apoptosis was analyzed by flow cytometry. E and F, Transwell assay was used for cell migration and invasion analysis in MG-63 and U2OS cells. Data are reported as means±SD. **P<0.01 (Student's t-test and ANOVA).
