Figure 5. EPHA7 was a target of miR-101. A, Predicted binding sites between miR-101 and EPHA7 3'UTR. B, The expression of EPHA7 was measured through qRT-PCR assay in osteosarcoma (OS) tissues and adjacent normal tissues. C, Correlation analysis between miR-101 expression and EPHA7 expression in OS tissues. D and F, Relative luciferase activity was measured in MG-63 and U2OS cells cotransfected with miR-101 mimics and EPHA7-WT (wild-type) or EPHA7-MUT (mutant). E and G, EPHA7 protein expression level was observed by western blot in MG-63 and U2OS cells with NC (negative control) mimics, miR-101 mimics, NC inhibitor, or miR-101 inhibitor transfection. Data are reported as means±SD. **P<0.01 (Student's t-test and ANOVA).
