Figure 6. HCP5 regulated cell proliferation, apoptosis, migration, and invasion in osteosarcoma (OS) cells by controlling the expression of EPHA7 via targeting miR-101. A and B, Relative protein expression level of EPHA7 was determined by western blot in MG-63 and U2OS cells transfected with si-NC (negative control), si-HCP5#2, si-HCP5#2+miR-101 inhibitor, miR-101 inhibitor+si-NC, and miR-101 inhibitor+si-EPHA7. C and D, Cell proliferation was measured by MTT assay in MG-63 and U2OS cells containing diverse transfection. E, Cell apoptosis was examined using flow cytometry. F and G, Cell migration and invasion were detected in MG-63 and U2OS cells after transfection by transwell assay. Data are reported as means±SD. **P<0.01 (ANOVA).
