Fig. 2.

Androgen repressed the transcription of hundreds of genes and PVT1 knockdown partially reversed this repression in LNCaP cells. LNCaP cells in culture were deprived of androgen (-R1881) or stimulated with 1 nM androgen (+ R1881), RNA was extracted from four biological replicates, purified and used. a Detection with microarrays of genome-wide statistically significant gene expression changes (one replicate in each column, one gene in each line; z-core color scale on the left) (q-value < 0.01, fold-change < 0.5 or fold-change > 2). b Validation with RT-qPCR of changes in expression of known androgen-responsive genes. c PVT1 knockdown with a pool of two antisense LNA GapmeRs (PVT1 KD) was efficient both in the presence (red) or in the absence (blue) of androgen, compared with a scrambled GapmeR (CTRL KD). d RNA was extracted from LNCaP cells after PVT1 knockdown or CTRL knockdown and used for detection with microarrays of genome-wide statistically significant gene expression changes induced by PVT1 knockdown (q-value < 0.01, fold-change < 0.5 or fold-change > 2). e Venn diagram shows that 160 genes were both downregulated by androgen and upregulated after PVT1 knockdown under androgen stimulation. T-test, *P < 0.05, **P < 0.01, ****P < 0.001; mean ± s.e.m., four biological replicates