Fig. 5.

Epigenetic marks at the enhancer and promoter genomic regions of the NOV gene were remodeled upon PVT1 knockdown. LNCaP cells in culture with androgen were treated with a pool of two antisense LNA GapmeRs targeting PVT1 (PVT1 KD) or with a scrambled GapmeR (CTRL KD), cells were then lysed and submitted to the Chromatin Immunoprecipitation (ChIP) protocol. a, b ChIP with anti-H3K27me3 Ab; or c, d with anti-H3K27ac Ab. The co-precipitated DNA was subjected to qPCR with primers for the enhancer (left two bars) and promoter (right two bars) genomic regions of the gene indicated at the bottom of each panel, either the NOV or the PSA gene. T-test, *P < 0.05, mean ± s.e.m. of three biological replicates