Fig. 5.

Removal of total biomass for the CFU count by means of scraping or both scraping and ultra-sonication. A) 3D projections of remaining biomass after thorough scraping. B) 3D projections of remaining biomass after thorough scraping followed by ultra-sonication. Images consist of 7 × 7 stitched z-stack images with a 100x magnification. C) Quantitative measurements of biomass left in the wells after scraping or both scraping and ultra-sonication, compared to the undisturbed control. D) The number of CFUs removed by scraping alone, ultra-sonication alone, or by the two methods combined.