Fig. 6.

Representative 3D projections of 24-h old biomass remaining at the bottom of microtiter wells after the supernatant had been removed as part of the crystal violet staining procedure, by means of inversion, pipetting, or pipetting followed by a rinse with saline. Images consist of 7 × 7 stitched z-stack images with a 100x magnification. A–B) Biomass remaining after the removal of the supernatant by means of inversion. C–D) Biomass remaining after removal of the supernatant by means of pipetting. E–F) Biomass remaining after rinsing with saline. G) Quantitative measurements of 24-h old biomass either undisturbed or remaining at the bottom of microtiter wells after the supernatant had been removed as part of the crystal violet staining procedure, by means of inversion, pipetting, or pipetting followed by a rinse with saline. H) OD₅₉₅ measurement of crystal violet staining of remaining biomass after the supernatant was removed either by inversion or pipetting followed by a rinse with saline. I) CFU count for the biomass in undisturbed wells or remaining in the wells after inversion or pipetting and rinse. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)