Table 2.
Composition of salt, trace mineral and vitamin mixtures used in “Ca. L. asiaticus” growth medium.
| 100 × Salt mixture | Concentration (g/L) |
|---|---|
| KCl | 38.0 |
| NH4Cl | 20.0 |
| NaH2PO4 · H2O | 6.9 |
| CaCl2 · 2H2O | 4.0 |
| MgSO4 · 7H2O |
20.0 |
|
100 × Trace mineral mixture |
g/L |
| Nitrilotriacetic acid (NTA) | 1.500 |
| - pH NTA solution to 10, then add: | |
| MnCl2 · 4H2O | 0.100 |
| FeSO4 · 7H2O | 0.300 |
| CoCl2 · 6H2O | 0.170 |
| ZnCl2 | 0.100 |
| CuSO4 · 5H2O | 0.040 |
| AlK(SO4)2· 12H2O | 0.005 |
| H3BO3 | 0.005 |
| Na2MoO4 | 0.090 |
| NiCl2 | 0.120 |
| NaWO4· 2H2O | 0.020 |
| Na2SeO4 | 0.100 |
| - pH solution to 7.0 | |
|
100 × Vitamin mix |
mg/L |
| Biotin | 2.0 |
| Folic acid | 2.0 |
| Pyridoxine hydrochloride | 10.0 |
| Riboflavin | 5.0 |
| Thiamine hydrochloride | 5.0 |
| Nicotinic acid | 5.0 |
| DL-calcium pantothenate | 5.0 |
| Vitamin B12 | 0.1 |
| p-Aminobenzoic acid | 5.0 |
| Thiocidic (lipoic) acid | 5.0 |