Fig. 5.

Co-culture colonies on 0.6% agar consisting of P. aeruginosa PAO1 with P. aeruginosa small colony variant (SCV) (A, C), and K. pneumoniae with K. pneumoniae KP-1 non-mucoid variant (NMV) (B, D), overlap between differently labeled strains (E) and the area covered by individual species/strains (F). Each species was labeled with different fluorescent protein genes and are presented here as yellow (P. aeruginosa wild-type), magenta (P. aeruginosa SCV), red (K. pneumoniae wild-type) and cyan (K. pneumoniae NMV). Strains were mixed 1:1 (OD₆₀₀) at the time of inoculation and colonies were imaged after 24 and 48 ​h of growth. Scale bar indicates 1000 ​μm. Boxplots are Tukey style. Paired letters indicate statistically significant differences between c and d (but not c and a, see previous figures) (p ​≤ ​0.05) according to ANOVA and Tukey’s honest significant differences test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)