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. 2019 Jun 15;1:100001. doi: 10.1016/j.bioflm.2019.100001

Table 1.

Strains and plasmids used in the present study. TpR: trimethoprim resistance marker, CmR: chloramphenicol resistance marker.

Strain Information Source (reference)
B. cenocepacia
J2315 (LMG16656) CF patient, UK BCCM/LMG Bacteria Collection
Overexpression mutants
Vector control J2315 pScrhaB2 empty vector, TpR [27]
J2315 pScrhaB2 BCAL0008a, TpR This study
J2315 pScrhaB2 BCAL0683, TpR This study
J2315 pScrhaB2 BCAL2532, TpR This study
J2315 pScrhaB2 BCAL2734, TpR This study
J2315 pScrhaB2 BCAL3186, TpR This study
J2315 pScrhaB2 BCAM0271, TpR This study
J2315 pScrhaB2 BCAM0971, TpR This study
J2315 pScrhaB2 BCAM2623, TpR This study
J2315 pScrhaB2 pBCA050, TpR This study
eGFP translational fusion reporters
Vector control J2315 pJH2 empty vector, CmR [24]
J2315 pJH2 BCAL0008a, CmR This study
J2315 pJH2 BCAL0683, CmR This study
J2315 pJH2 BCAL2523, CmR This study
J2315 pJH2 BCAL2734, CmR This study



J2315 pJH2 BCAM0271, CmR This study



J2315 pJH2 BCAM2623, CmR This study



E. coli DH5α BCCM/LMG Bacteria Collection
E. coli DH5α BCAM0271-2 pScrhaB2 BCAM0271-2, TpR This study
E. coli helper pRK2013 [26]
E. coli DJ624Δara BCAM0272 pBAD33 BCAM0272 This study
E.coli DJ624Δara MG1655, lac Iq, Δara [44]
Plasmids
pBAD33 P15A, CmR, pBAD promoter [44]
pJH2 promoter eGFP replaced with multiple cloning site, CmR [24]
pScrhaB2 oripBBR1rhaR, rhaS, PrhaB TpRmob+ [27]