B. cenocepacia |
J2315 (LMG16656) |
CF patient, UK |
BCCM/LMG Bacteria Collection |
Overexpression mutants |
Vector control |
J2315 pScrhaB2 empty vector, TpR
|
[27] |
|
J2315 pScrhaB2 BCAL0008a, TpR
|
This study |
|
J2315 pScrhaB2 BCAL0683, TpR
|
This study |
|
J2315 pScrhaB2 BCAL2532, TpR
|
This study |
|
J2315 pScrhaB2 BCAL2734, TpR
|
This study |
|
J2315 pScrhaB2 BCAL3186, TpR
|
This study |
|
J2315 pScrhaB2 BCAM0271, TpR
|
This study |
|
J2315 pScrhaB2 BCAM0971, TpR
|
This study |
|
J2315 pScrhaB2 BCAM2623, TpR
|
This study |
|
J2315 pScrhaB2 pBCA050, TpR
|
This study |
eGFP translational fusion reporters |
Vector control |
J2315 pJH2 empty vector, CmR
|
[24] |
|
J2315 pJH2 BCAL0008a, CmR
|
This study |
|
J2315 pJH2 BCAL0683, CmR
|
This study |
|
J2315 pJH2 BCAL2523, CmR
|
This study |
|
J2315 pJH2 BCAL2734, CmR
|
This study |
|
|
J2315 pJH2 BCAM0271, CmR
|
This study |
|
|
J2315 pJH2 BCAM2623, CmR
|
This study |
|
E. coli DH5α |
|
BCCM/LMG Bacteria Collection |
E. coli DH5α BCAM0271-2 |
pScrhaB2 BCAM0271-2, TpR
|
This study |
E. coli helper |
pRK2013 |
[26] |
E. coli DJ624Δara BCAM0272 |
pBAD33 BCAM0272 |
This study |
E.coli DJ624Δara |
MG1655, lac Iq, Δara |
[44] |
Plasmids |
pBAD33 |
P15A, CmR, pBAD promoter |
[44] |
pJH2 |
promoter eGFP replaced with multiple cloning site, CmR
|
[24] |
pScrhaB2 |
oripBBR1rhaR, rhaS, PrhaB TpRmob+ |
[27] |