Fig. 7.

Colocalization analysis of functionalized SiNPs and differentially-labelled EPS components within the biofilm matrix. Fiji’s JaCoP colocalization analysis (Mander’s correlation coefficients) was conducted to examine pixel to pixel correlation in separate channels over space in several z-stack CLSM images. Data is representative of the mean ​± ​standard deviation of three separate experiments, each of which contained at least 3 z-stack CLSM images. A one-way ANOVA, Tukey’s pairwise comparison, (P ​< ​0.05) was used to assess statistical differences.