Fig. 2.

Sialyl Tn is not expressed on lung and breast cancer cell lines that bind Siglec-15-Fc. Expression of sTn on lung (A) and breast (B) cancer cell lines was detected by anti-sTn antibody (clone 3F1) (7.5 μg/mL) followed by APC conjugated anti-mouse IgG. An isotype matched mouse IgG was used as the negative control. The results are plotted as median fluorescence intensities ± SEM from 3 independent experiments, relative to the signal with secondary antibody alone. ^***, P < 0.005; ^****, P < 0.001; ns, not significant.