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. Author manuscript; available in PMC: 2022 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2020 Dec 4;521:111110. doi: 10.1016/j.mce.2020.111110

Figure 1.

Figure 1.

Chronic rotating shiftwork schedule for 6 months promotes adipocyte hypertrophy in adipose depots. (A) Schematic representation of weekly rotating lighting shift regimen in the shiftwork group with normal L/D from Monday to Thursday and reversed L/D cycle from Friday to Sunday. Two consecutive weeks were shown and the weekly rotation was maintained for 24 weeks. Samples were collected at indicated 8AM and 5PM on Tuesday with normal L/D lighting for both normal and shiftwork groups. (B) Body weight of normal control and shiftwork group mice at beginning of shift regimen and after 6 months (n=12/group). (C) Representative H/E histology after 6 months of shiftwork, and (D) histogram of adipocyte size distribution of eWAT (n=6/group). (E) Representative H/E histology and (F) adipocyte size distribution of iWAT (n=6/group). For adipocyte size measurement, three 10X fields were counted in each sample of normal or shiftwork groups (n=6). *: P≤0.05 or **: P≤0.01 by ANOVA.